棰? ???Noncanonical RNA caps
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涓昏?浜虹?浠?锛?
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璁插骇绠?浠?锛?
In eukaryotes, messenger RNAs (mRNAs) harbor a 5?? methylguanosine (m7G) cap, which stabilizes mRNAs and assists with their processing, nuclear export and translation. In recent years, it has come to be realized that the metabolite and redox agent, NAD+/NADH, can serve as an RNA cap in prokaryotes and all major lineages of eukaryotes (plants, fungi, and animals). We and others showed that NAD+-capped RNAs (NAD-RNAs) are widespread in Arabidopsis and are largely mRNAs from the nuclear and mitochondrial genomes. We found that Arabidopsis NAD-RNAs are spliced and polyadenylated and are associated with polysomes. I will discuss our work on the development of various transcriptomic strategies to identify and quantify NAD-RNAs with specificity and sensitivity. I will also discuss decapping mechanisms that regulate the levels of NAD-RNAs. I will touch upon our efforts in developing technologies to profile RNAs capped with other cellular metabolites such as FAD and dpcoA.